Scientific Program

Day 1 :

  • Nanotherapeutics and Diagnosis | Pharmaceutical Nanotechnology | Nanoparticle Based Drug Delivery

Session Introduction

Henrique Faneca

Principal investigator University of Coimbra Portugal

Title: Development of novel nanosystems to mediate combined and multi-target antitumor therapeutic strategies
Speaker
Biography:

Henrique Faneca is principal investigator at Centre for Neuroscience and Cell Biology, and invited assistant professor at University of Coimbra. He received his Ph.D. degree in Biochemistry from Coimbra University in 2005. The main focus of his research is the development of lipid- and polymeric-based nanosystems for gene and drug delivery into target cells and the generation of new antitumor strategies, involving different gene therapy approaches either per se or in combination with chemotherapeutic agents. Henrique Faneca is author of more than 45 scientific papers corresponding to over 1500 citations and to an h-index of 19.

Abstract:

Cancer is one of the major causes of death, since conventional available treatments, in most of the cases, do not allow a cure of the disease. Despite the ongoing efforts, current treatment options are associated to multiple limitations, including reduced therapeutic efficacy and high side effects. The lack of effective and well-tolerated cancer treatments highlights the urgent need for the development of new therapeutic approaches, such as those involving the combination of gene therapy and chemotherapy. However, the synchronized application of these two types of strategies requires the development of efficient delivery nanosystems, in order to promote an effective and specific delivery of both therapeutic molecules into tumors, while avoiding their release in healthy tissues.  

In this context, we have recently developed new gene delivery nanosystems, polymer-based ones that have the ability to condense and efficiently deliver genetic material, and lipid-based ones that have the ability to specifically and efficiently deliver genetic material into HCC cells both in vitro and in vivo. Moreover, we have also shown that combination of antitumor gene therapy strategies, such as those including therapeutic genes or anti microRNA oligonucleotides, with low amounts of chemotherapeutic agents could result in a synergistic and significant antitumor effect.

Our present and future work will be focused on the engineering and characterization of novel nanoparticles, for drug and gene delivery, to mediate innovative multi-target antitumor strategies involving the combination of gene therapy and chemotherapy, in order to achieve a much higher antitumor efficacy and less side effects than conventional therapeutic strategies.

 

Speaker
Biography:

Ziyad S. Haidar is a Full Professor of Biomaterials and Tissue Engineering and the Scientific Director of the Faculty of Dentistry, Universidad de los Andes in Santiago de Chile. Concurrently, he is the Founder and Head of the Biomaterials, Pharmaceutical Delivery and Cranio-Maxillo-Facial Tissue Engineering Laboratory/Research Group (BioMAT'X Chile; please visit: http://www.uandes.cl/facultad-de medicina/biomaterials-pharmaceutical.html). Haidar also serves as the Head of Innovation at the Centro de Investigación e Innovación Biomédica and a Faculty member in the Doctoral Program (BioMedicine) at the Facultad de Medicina. He is a Visiting Professor at several institutions, such as the Division of MaxilloFacial Surgery at the Universidad de la Frontera in Temuco. Haidar is a trained dentist, implantologist and an oral and maxillofacial surgeon with a PhD in nanobiomaterials, pharmaceuticals and tissue engineering from McGill University, Montréal, Canada. He is an international speaker with more than 125 publications, conference proceedings, text-books and patents and is an editorial board member of several national and international peer-reviewed scientific journals / periodicals. 

Abstract:

Statement of the Problem: Saliva plays a major role in maintaining oral health. This becomes more apparent when the amount and quality of saliva are reduced, often due to medications, Sjögren’s syndrome and especially ionizing radiation therapy for tumors of the head and neck, during which the salivary glands are included within the radiation zone. While temporarily alleviated via “intensive” regimens of palliative home and professional care, many Head and Neck Cancer patients are unable to maintain the diligence required to be effective. More considerably, those affected by irreversible salivary gland dysfunction (and/or using amifostine, I.V.) often choose to terminate their radiotherapy course pre-maturely as they become severely malnourished and experience a significant deterioration in their QoL, mainly owing to hyposalivation.

Aims: Evaluate the radioprotective effect of core-shell nanocapsules designed for sequential/timely protein(s) release, following a single local injection into murine submandibular salivary glands pre-irradiation.

Materials and Methods: Loaded core-shell nanocapsules with the protein(s) were directly administered into the salivary glands of the experimental group 24 hours before radiation and PBS was injected into the glands, likewise, for the controls. Salivary flow rates and salivary protein excretion/content were evaluated using ELISA over a 3mons. period post-treatment. Histological evaluation of structures and analysis of apoptosis/proliferation were performed. Timely bio-distribution assays followed.

Findings: Experimental animals demonstrated increased salivary flow rates compared to controls. Protein content was comparable to that of pre-radiation level. Histological evaluation revealed acinar cells with less vacuoles and nuclear aberrance in experimental group compared to controls and the amount of mucin stained by alcian blue was larger, in the latter. Protein therapy resulted in less apoptotic activities detected by TUNEL assay and similar proliferative indices as in controls.

Conclusion & Significance: Biocompatible, stable, reproducible and customizable core-shell nanoparticulate layer-by-layer self-assembled delivery system is presented. Our findings suggest that the local sequential release of a protein cocktail (in specific dosage and order) into murine salivary gland highly prevents radiation-induced damage via reducing apoptosis. This approach also promotes the in situ proliferation of salivary gland cells.Image.

 

Speaker
Biography:

Abstract:

According to the world health organization, China surpassed the United States as has the largest population of obese in the world in 2015. In China, the overall prevalence of adult dyslipidemia is as high as 40.40%, about 160 million patients. Dyslipidemia is an important risk factor for atherosclerotic cardiovascular disease (ASCVD). In recent years, the purpose of the treatment of blood lipids in the world is to prevent and control ASCVD, and reduce cardiovascular events such as myocardial infarction, stroke or death from coronary heart disease. Over the past 20 years, a number of large-scale clinical trial results consistently show that statin can significantly reduce the cardiovascular event risk in the primary and secondary prevention of ASCVD. In order to achieve the ideal blood lipid, statin was the first choice in clinic. Increasing evidences showes that high-intensity statin therapy is associated with higher risk of myopathy and liver enzymes, which is more prominent in the Chinese population. HPS2-THRIVE studies showed that the incidence of hepatic adverse reactions in Chinese patients was significantly higher than that in European patients when using moderate-intensity statin therapy. At present, there is no safety data on the high intensity statin treatment of Chinese population.

In order to solve the present situation of dyslipidemia in China, we researched and developed the Daming capsule (patent number: ISSN1008-4274, the national drug approval number: Z20030085). Daming capsule is composed of six traditional Chinese herb containing rhubarb, semen cassiae, salvia miltiorrhiza, ginseng, tangerine peel, and poria. It is used to treat hyperlipidemia in clinic. Six randomized multicenter clinical trails have verified that Daming capsule can significantly reduce serum TC and LDL-c contents in patients with hyperlipemia. In addition, Daming capsule also has the effect of lowering blood glucose and protecting the heart function of diabetic rats. The pharmacological action of Daming capsule may be related to the up-regulation of GLP-1 protein and beta-catenin, as well as the elevation of Cav1.2, Kv4.2 and connextin 43.

In order to specify the bioactive components of Daming capsule, we first developed a high-throughput, high-resolution, and high-sensitivity ultra high performance liquid chromatography with electrospray ionization quadrupole time-of-flight tandem mass spectrometry method. The established strategy based on ultra high performance liquid chromatography with electrospray ionization quadrupole time-of-flight tandem mass spectrometry was successfully applied to screen the bioactive components of Daming capsule. Up to 53 absorbed compounds were identified. Six anthraquinones with fast and high absorption, namely, emodin-O-glucoside, aurantio-obtusin, aloe-emodin, rhein, emodin, and chrysophanol, were screened as potentially bioactive components of Daming capsule.

At present, we are working on the selection and optimization of these bioactive components, and we have made a new safe and effective hypolipidemic derivative. we hope that the discovery of this drug can solve high prevalence of dyslipidemia in China.

 

  • Nanomaterials and Nanoparticles | Medical Nanorobotics | Medical Nanomaterials and Nanodevices

Session Introduction

Muaathe A. Ibraheem

INSTRUCTOR at COLLEGE OF SCIENCE-UNIVERSITY OF DIAYLA

Title: Study of Polypyrrole-coated electrospun polystyrene fibres by electrochemical oxidization
Speaker
Biography:

Abstract:

Polypyrrole (Ppy) was synthesized via a chemical oxidization method with FeCl3.6H2O (FCHH) on electrospun polystyrene fibres ESPSF. The spinning process was achieved by means of Electrospinning for three solutions of polystyrene PS including 3, 5 and 9 % wt/v of FCHH in dimethyl formamide (DMF). The fibres diameters were monitored  by scanning electron microscope SEM before and after coating and it was found that best results were achieved at low concentration of FCHH. Coating with Ppy has been performed in four exposure periods (90, 150, 200, 270 min) in a temperature was at 22C and the results show a direct relation between the mean diameter of fibres with quality of coating. The technique suggests a potential route towards the production of conducting fibre with micro/nanoscale dimensions  

Key words: Pyrrole, Electrospinning, polystyrene, conducting polymer & Fibres.

Speaker
Biography:

Abstract:

In this report, we present a facile approach to forming dendrimer-functionalized LAPONITE® (LAP) nanodisks loaded with gadolinium (Gd) for in vitro and in vivo T1-weighted MR imaging applications. In this work, LAP nanodisks were sequentially modified with silane coupling agents, succinic anhydride to have abundant carboxyl groups on their surface, and amine-terminated poly(amidoamine) (PAMAM) dendrimers of generation 2 (G2). The dendrimer-modified LAP nanodisks were then conjugated with gadolinium (Gd) chelator diethylenetriaminepentaacetic acid (DTPA), followed by Gd(III) chelation to form the LM–G2–DTPA (Gd) nanocomplexes. The designed LM–G2–DTPA(Gd) nanocomplexes were characterized via different techniques. Cell viability assay shows that the formed LM–G2–DTPA(Gd) nanodisks are non-cytotoxic in the given concentration range. With a high r1 relaxivity (2.05 mM1 s1), the LM–G2–DTPA(Gd) nanocomplexes are able to be used as an efficient contrast agent for T1-weighted MR imaging of cancer cells in vitro and animal organ/tumor model in vivo. The designed LM–G2– DTPA(Gd) nanocomplexes may hold a great promise to be used as a versatile nanoplatform for MR imaging of different biological systems.

Speaker
Biography:

Abstract:

Formation of micro- and nano-droplets and their novel applications are of continued and broad interest. In this paper, the method that utilizes MHz Faraday waves driven by centimeter-sized multiple Fourier horn ultrasonic nozzles and the underlying science for formation of monodisperse micron-sized droplets and the resulting pocket-size nebulizers (Technology [2, 75-81, 2014]) are reported. Imminent application of the new technology to inhalation drug delivery and potential applications of the micron-sized droplets such as formation of nanoparticles via spray drying and spray pyrolysis are also discussed

Febee R. Y. Louka

Associate professor UNIVERSITY OF LOUISIANA AT LAFAYETTE United States

Title: Nano- and Micro sensors in Determination of Cardiovascular System Lifespan
Speaker
Biography:

Dr. Louka holds a Ph.D. in Medicinal Analytical Chemistry (2004) from Ohio University, Athens OH. Currently, she is an associate professor in the Chemistry Department at the University of Louisiana at Lafayette. Her expertise is in environmental and medicinal analytical chemistry. She has accumulated over 25 years of teaching and research experience in instrumental analysis and has been certified by “Monitoring the environmental pollutants” by the [Marine Environmental Laboratories of the International Atomic Energy Agency in Monaco].

Dr. Louka started to focus on a new point of interest, by using biological backbone synthesized surfactants in drug delivery especially, for cancer cells and DNA cleavage. She is and will be investigating new inexpensive methods and eco-friendly adsorbents from Louisiana waste products and biological backbone synthesized surfactants.

Currently, Dr. Louka is the primary operator for all instruments in the analytical laboratories. She has extensive experience in the development of techniques and refinement of existing techniques of instrumental analysis. She was awarded the Outstanding Teaching Award College of Sciences 2016. She has published over 65 articles and presentations in peer-reviewed journals and conferences. She is a member of the American Chemical Society. She was one of four professors chosen from the whole university who established and initiate the Undergraduate Research Council. She has mentored more than 75 undergraduate students and two graduate students.

Nowadays, most of students under her supervision presented their results in the ACS meeting; others are co-authors in publications in highly ranked journals or professional meetings. A student in her undergraduate students’ research group was awarded the American Chemical Society Undergraduate Outstanding Analytical Chemist Award (2015). Dr. Louka was the awardee of Outstanding Undergraduate Research Mentoring 2014 and the Marvin and Warren Boudreaux / BoRSF Professorship in Chemistry 2012 – 2015 and 2015-2018. In 2016, She was the awarded Outstanding Teaching Award College of Sciences. She is also a member of the honors program which take the students to a level higher than regular students, preparing them for graduate, medical, pharmacy, and dental schools.

 

 

Abstract:

 

The imbalance between pro-oxidative and anti-oxidative processes increases with age. Dysfunctional endothelium is associated with impaired generation of nitric oxide (•NO) and overproduction of superoxide (O2•¯). This work describes the study of the relationship between the degree of endothelial dysfunction and the lifespan of the cardiovascular system. Ames dwarf, transgenic giant (Tg), hypertensive (SHR) and normal mice were used for these studies. The dwarf mice appear to outlive their normal siblings by an average of at least one year. However, the Tg mice have an over-expressed growth hormone that stimulates the growth of their bodies. One of the most important features of these mice is their reduced life expectancy.

An array of •NO, O2•¯ and peroxynitrite (ONOO¯) electrochemical nanosensors was used for the in vitro measurements. The balance between •NO, O2•¯ and ONOO¯ formations in Ames, giant and normal mice as well as SHR rats were investigated in their cardiovascular systems.

More favorable kinetics of •NO production with a concurrent quenched O2•¯ release was revealed in Ames compared to normal mice. The •NO/O2•¯ peak ratio was found to be 3.0 ± 0.29 times higher for Ames dwarf than their normal siblings. The case of transgenic mice was reversed, where the •NO/O2•¯ ratio was 2.8 ± 0.22 times less than that of controls from same Tg line. The ONOO¯ release was also determined in all species.

The rate of •NO production decreased from 1.2 + 0.1 μmol s-1 in WKY to 0.46 + 0.04 μmol s-1 in the SHR rats. Also, maximum •NO concentration in SHR was found to be much lower than that in WKY. On the other hand, the O2•¯ and ONOO¯ concentrations dramatically increased in SHR compared to WKY. Therefore, the change in dynamics of •NO release in the dysfunctional endothelium can be attributed to the increase in generation of O2•¯ as well as that of ONOO¯. Our data indicated that the reduced lifespan in Tg mice with dysfunctional endothelium is associated with high concentrations of O2•¯ and ONOO¯ that most likely leads to accumulation of tissue oxidative damage.

 

  • Intelligent Biomaterials and Smart Implants | Nanomedicine in Cancer Therapeutics | Nanofiber Based Scaffolds and Tissue Engineering

Session Introduction

Babak Negahdari

Associate Professor TUMS Iran

Title: Nanoparticles as new tools for inhibition of cancer angiogenesis
Speaker
Biography:

Babak Negahdari has his expertise in medical biotechnology and nanotechnology. His experimental projects are based on application of nanotechnology and biotechnology in life improving of cancer patients and their therapeutic applications.

 

Abstract:

Angiogenesis is known as one of the hallmarks of cancer. Multiple lines evidence indicated that vascular endothelium growth factor (VEGF) is a key player in the progression of angiogenesis and exerts its functions via interaction with tyrosine kinase receptors (TKRs). These receptors could trigger a variety of cascades that lead to the supply of oxygen and nutrients to tumor cells and survival of these cells. With respect to pivotal role of angiogenesis in the tumor growth and survival, finding new therapeutic approaches via targeting angiogenesis could open a new horizon in cancer therapy. Among various types of therapeutic strategies, nanotechnology has emerged as new approach for the treatment of various cancers. Nanoparticles (NPs) could be used as effective tools for targeting a variety of therapeutic agents. According to in vitro and in vivo studies, NPs are efficient in depriving tumor cells from nutrients and oxygen by inhibiting angiogenesis. However, the utilization of NPs are associated with a variety of limitations. It seems that new approaches such as NPs conjugated with hydrogels could overcome to some limitations. In the present review, we summarize various mechanisms involved in angiogenesis, common anti-angiogenesis strategies, and application of NPs for targeting angiogenesis in various cancers.

 

Recent publications (minimum 5)

1. Nanoceria: a rare-earth nanoparticle as a novel anti-angiogenic therapeutic agent in ovarian cancer. PloS one 8(1):e54578

2. Gurunathan S, Lee K-J, Kalishwaralal K, Sheikpranbabu S, Vaidyanathan R, Eom SH. 2009. Antiangiogenic properties of silver nanoparticles. Biomaterials 30(31):6341-6350.

3. Jin H, Pi J, Yang F, Wu C, Cheng X, Bai H, Huang D, Jiang J, Cai J, Chen ZW. 2016. Ursolic acid-loaded chitosan nanoparticles induce potent anti-angiogenesis in tumor. Applied microbiology and biotechnology 100(15):6643-6652.

4. Hu H, You Y, He L, Chen T. 2015. The rational design of NAMI-A-loaded mesoporous silica nanoparticles as antiangiogenic nanosystems. Journal of Materials Chemistry B 3(30):6338- 6346.

5. Guarnieri D, Malvindi MA, Belli V, Pompa PP, Netti P. 2014. Effect of silica nanoparticles with variable size and surface functionalization on human endothelial cell viability and angiogenic activity. Journal of nanoparticle research 16(2):2229.

 

 

 

Speaker
Biography:

Ziyad S. Haidar is a Full Professor of Biomaterials and Tissue Engineering and the Scientific Director of the Faculty of Dentistry, Universidad de los Andes in Santiago de Chile. Concurrently, he is the Founder and Head of the Biomaterials, Pharmaceutical Delivery and Cranio-Maxillo-Facial Tissue Engineering Laboratory/Research Group (BioMAT'X Chile; please visit: http://www.uandes.cl/facultad-de- medicina/biomaterials-pharmaceutical.html). Haidar also serves as the Head of Innovation at the Centro de Investigación e Innovación Biomédica and a Faculty member in the Doctoral Program (BioMedicine) at the Facultad de Medicina. He is a Visiting Professor at several institutions, such as the Division of MaxilloFacial Surgery at the Universidad de la Frontera in Temuco. Haidar is a trained dentist,  implantologist and an oral and maxillofacial surgeon with a PhD in nanobiomaterials, pharmaceuticals and tissue engineering from McGill University, Montréal, Canada. He is an international speaker with more than 125 publications, conference proceedings, text-books and patents and is an editorial board member of several national and international peer-reviewed scientific journals / periodicals. 

Abstract:

Statement of the Problem: Paclitaxel (PAX) is a chemotherapy agent; effective in the treatment of a broad range of human malignancies. PAX has an anti-angiogenic activity through inhibiting vascular endothelial cell proliferation, motility and cord formation, at extremely low concentrations. Yet, side effects including hypersensitivity reactions, neurotoxicity, cardiotoxicity, and nephrotoxicity, are common, and are carrier-related/(CrEL). Multi-drug resistance (MDR) resulting in

chemotherapeutic failure has been reported. Cost is an issue as well. Hence, it is necessary to develop an alternative formulation fit for controlled PAX delivery. SLN (solid lipid nanoparticles) as potential anticancer drug delivery nanocarriers, exhibit a great superiority to modulate drug release, improve anti-cancer activity and overcome MDR. We developed a novel natural polymer-lipid hybrid formulation consisting of SLN as core and chitosan-hyaluronic acid (CH-HA) as a shell, with HA as the outmost layer; to enhance selectivity towards HA receptors in MCF-7 cells.

Aims: To investigate the potential of modified SLN for the delivery of PAX.

Materials and Methods: SLN loaded with PAX were prepared via high-pressure hot homogenization. Formulation parameters were optimized to obtain a high-quality delivery system. Selectivity towards HA receptors was tested in a breast cancer cell line.

Findings: Stable, reproducible and positively-charged nanoparticles

resulted. Findings reveal that CH-HA-coated SLN facilitated the targeting, cellular uptake and the time-/dose-controlled

delivery/release of PAX, enhancing intrinsic chemotherapeutic activities. CH exhibits an increased uptake efficiency by negatively-charged cancer cell membranes due to electrostatic interactions. HA is a bioadhesive compound capable of binding with high affinity to cell surface and intracellular receptors.

Conclusion & Significance: SLN are suitable carrier candidates for nanoncology given their localized, and potent cytotoxic potential overcoming MDR cancer cells. CD44, an HA receptor, is overexpressed in cancer stem cells, therefore, targeting CD44 using HA seems as a fine strategy to eliminate cells accountable for treatment failure and cancer recurrence.

  • Impact of Nanomedicine on Health Care | Future Concepts in Nanomedicine | Ethical and Social Aspects of Nanomedicine

Session Introduction

Roland H. Stauber

University Medical Center of Mainz Germany

Title: PATHOGEN-NANOMATERIAL CROSSTALK: PRINCIPLES AND RELEVANCE OF NANOANTIBIOTICS
Speaker
Biography:

Abstract:

Nanomaterials have dramatically increased in functionality and complexity, potentially allowing also unprecedented control over microbial pathogens, such as bacteria, fungi, or viruses. From these advances arises the prospect of novel nanomaterial-based antimicrobial therapies, which clearly differ from their bulk counterparts. Yet, practi­cal constraints require a rational understanding of NMs' physico-chemical characteristics and parameters at the nano-bio interface that impact their antimicrobial activity. We here define the most important nanomaterial parameters and biological outputs enabling controllable antimicrobial activity, while minimizing the complexity of designs. We present the problematic of nanomaterial resistance and mechanistic strategies to overcome these current limitations for next-generation practical applications. The presented insights will inspire nanomaterial designs that maximize functionality and safe translatability as new nanoantibiotics, and deepened our understanding on the biomedical and ecological relevance of nanomaterial-microbiota cross-talk in general.

 

Day 2 :

  • Nanomaterials and Nanoparticles | Medical Nanorobotics | Medical Nanomaterials and Nanodevices
Speaker
Biography:

Abstract:

Diabetes is a defect in the body’s ability to convert excess glucose to glycogen. Two classes of antidiabetic drugs were identified and nateglinide is a sub-division of oral antidiabetic drugs belonging to Meglitinides family. Gaussian 09 code which use density function theory as working principle was used to study the geometric, infrared, Raman, 1H-NMR, 13C-MNR spectrum and density of state of the nateglinide antidiabetic drug using exchange functional B3LYP/6-311G. For the geometry optimization, it was observed that, there is appearance of a new additional bond between C12 and N4 which changes the natural nature of both 1H-NMR and 13C-NMR spectra. For infrared and Raman spectra, functional groups and polarizability were observed. However, the result of density of state shows the large band gap of 5.46758eV and most of the orbitals were occupied at lower valance band.

Keywords: Nateglinide, Infrared, 1H-NMR, 13C-NMR, Density of state (DOS) and Raman Spectra.

 

Speaker
Biography:

Abstract:

Powdery and downy mildew can reduce both yield and quality of the fruit produced from affected grapes. In years with wet spring weather, downy mildew can cause widespread devastation, Powdery mildew has been estimated to cost the world grape and wine industry about $60 million each year. Downy mildew and  Powdery mildew may be controlled by a range of synthetic fungicides in conventional vineyards, synthetic fungicides are toxic to beneficial mites and insects, including natural antagonists and may contribute to environmental pollution and furthermore, fungicides residual are harmful to human health, the need to reduce environmental and ecological impacts in conventional viticulture, provide incentives for the development of alternative, environmentally friendly controls for powdery and downy mildew. The need to assess new, sustainable strategies for powdery mildew control on a range of cultivars in various climatic regions, to assess effects of these strategies on yield and  quality, to develop new materials for control of downy mildew was addressed in this research. Using of nano organic substance low molecular weight as a tool for controlling powdery and downy mildews. Experiment was carried out on superior grapevine cultivar during the seasons 2015-2016 to evaluate the efficacy of three concentrations of nano organic material of low molecular weight (NFA) to control downy and powdery mildew diseases compared with the recommended fungicides and their effects on plant growth, chlorophyll content, yield and quality of superior grapevines. Results revealed that all (NFA) concentrations significantly reduced disease severity of both diseases. The highest reduction in disease severity of downy mildew was recorded using 10 ppm of (NFA), which was more effective than the recommended fungicides. The significant effect of inhibition of powdery mildew diseases increased gradually with increased (NFA) concentration. At the same time, the reduction of diseases severity was greater than or equal to the recommended fungicides. Foliar application of (NFA) concentrations and synthetic fungicides increased significantly all morphological characters. The yield components (weight of bunch, berry weight, berry size, SSC, acidity and total sugar) recorded the same values with all (NFA) concentrations. NFA had direct effect on soil microbiology by increasing plant exudates, which enhanced the activity of microorganism. NFA at 15 ppm showed the highest increase in dehydrogenase and nitrogenase activity. This study revealed that the foliar application of (NFA) improved plant growth and yield quantity and quality as well as controlling powdery and downy mildews of superior grapevine cultivar. Additionally, NFA has the advantage as effective and environmental friendly agent (organic viticulture).The aim of the current research was to evaluate the use of Nano organic material extracted from wastes as an effective and low cost alternative controlling powdery and downy mildew diseases in grapevines, beside improving quantity and quality of yield and enhancing soil microbial activity, prevent environmental pollution and production of healthy organic food.

Keywords:   Environmental friendly, NFA, fungicides viticulture.

 

Speaker
Biography:

Abstract:

Sulfur-doped titanium oxide on the surface of sulfur-doped reduced graphene oxide nanocomposites (S-TiO2/S-RGO) were successfully synthesized for the first time through a simple low cost solvothermal reaction process. The sulfur doping was detected in both TiO2 matrix and carbon framework structure of reduced graphene oxide using X-ray photoelectron spectroscopy (XPS) and Energy-dispersive X-ray spectroscopy (EDX). Cross-sectional AFM analysis of S-RGO nanosheets reveals a thickness of 0.51 nm which is much thinner than those previously reported of heteroatom doped-RGO, confirming the single-layer feature. When the as-prepared (S-TiO2/S-RGO) nanocomposites are utilized as photoanodes for photoelectrochemical (PEC) water splitting, they exhibited an enhanced photoelectrochemical performance and long-term stability. The photocurrent density of S-TiO2/S-RGO(0.2) photoanode revealed 3.36 mA/cm2 at 1 V vs Ag/AgCl which is considered 3 times enhancement compared to bare synthesized TiO2. This enhancement in the photocurrent density was attributed to the increased separation rate of photogenerated electrons and holes and efficient visible light harvesting as a result of the successful combination of the S-TiO2 and the S-RGO in the same nanocomposite photoanode. This promising result presents a new approach for the synthesis of high-efficient future metal-free photoelectrocatalysts.

Keywords: S-TiO2; S-RGO; photoanode; Photoelectrochemical water splitting.

 

  • Nanomedicine in Neurodegenerative Disease | Nanotechnology and Surgery | Nanometrices in Cell Culture
Speaker
Biography:

Ziyad S. Haidar is a Full Professor of Biomaterials and Tissue Engineering and the Scientific Director of the Faculty of Dentistry, Universidad de los Andes in Santiago de Chile. Concurrently, he is the Founder and Head of the Biomaterials, Pharmaceutical Delivery and Cranio-Maxillo-Facial Tissue Engineering Laboratory/Research Group (BioMAT'X Chile; please visit: http://www.uandes.cl/facultad-de medicina/biomaterials-pharmaceutical.html). Haidar also serves as the Head of Innovation at the Centro de Investigación e Innovación Biomédica and a Faculty member in the Doctoral Program (BioMedicine) at the Facultad de Medicina. He is a Visiting Professor at several institutions, such as the Division of MaxilloFacial Surgery at the Universidad de la Frontera in Temuco. Haidar is a trained dentist, implantologist and an oral and maxillofacial surgeon with a PhD in nanobiomaterials, pharmaceuticals and tissue engineering from McGill University, Montréal, Canada. He is an international speaker with more than 125 publications, conference proceedings, text-books and patents and is an editorial board member of several national and international peer-reviewed scientific journals / periodicals. 

Abstract:

Statement of the Problem: Despite significant improvements, in reconstruction techniques and materials, during last decades, the regeneration, restoration and/or repair of oro-dental and maxillo-facial defects remains a challenge. Platelet Concentrates (PCs) are autologous blood extracts obtained through centrifugation of whole blood samples. The preparation procedure allows the gathering and concentration of platelets and other therapeutic blood constituents (fibrinogen/fibrin, growth factors, leukocytes and circulating cells), in clinically-usable preparations (surgical adjuvants), which may enhance, accelerate and promote tissue (hard and soft) wound healing and regeneration. Despite promising clinical observations, their overall effectiveness remains debated, to date. Today, it can be safely stated that, in oral and maxillofacial surgery, the Leukocyte and Platelet-rich Fibrin (L-PRF) sub-family is receiving the utmost attention, mainly due to simplicity, user-friendliness, malleability and potential cost-effectiveness, yet with outcome unpredictability.

Aims: Prepare, characterize, and evaluate a novel L-PRF bioscaffold incorporating human gingival mesenchymal stem cells and natural polymer-based nanoparticles capable to induce in situ healing and de novo local bone formation for its potential clinical application as a dental extraction socket preservation alternative strategy, immediately post-exodontia.

Materials and Methods: The resulting bioscaffold was characterized for its physico-chemico-mechanical and cytocompatibility/osteogenic properties, in vitro, followed by biocompatibility, malleability and efficacy, in vivo, in a challenging critical-sized defect model in the crania of normal WISTAR rats. Timely bio-distribution assays were performed. Histopathological, histomorphometrical and immunohistochemical evaluation and analysis followed.

Findings: Together, the results obtained show that is possible to formulate a safe, stable and biodegradable bioscaffold with intrinsic osteogenic potential as an advantageous alternative strategy for bone repair and regeneration.

Conclusion & Significance: Autologous L-PRF is often associated with early bone formation and maturation; accelerated soft-tissue healing; and reduced post-surgical pain, edema and discomfort. Indeed, measurements of volumen, density and area of the newly-formed bone in the defects together with the clinical observation of animals, showed a significant increase on the quantity and quality of the new bone when the defects were filled with the bio-scaffold, when compared to controls. A better analysis of rheological properties, bio-components and bioactive function of the nanoL-PRF preparation would enhance the cogency, comprehension and therapeutic potential of the reported findings; a step closer towards a new era of “super” oro-dental and maxilla-facial biomaterials and tissue engineering scaffolds.

 

  • Nanotherapeutics and Diagnosis | Pharmaceutical Nanotechnology | Nanoparticle Based Drug Delivery
Speaker
Biography:

Mrunal Patil has expertise in designing, formulating and evaluating lipid carriers for the treatment of fungal diseases. She has developed lipid-drug combination after years of learning fungal drug resistance patterns in K.E.M hospital and designing formulations at Institute of Chemical Technology. She has been awarded Newton Bhabha fellowship for carrying out research work in mechanism elucidation of active aminolipid carrier at Nottingham University, UK. She has a keen interest in design and developing safe, efficacious lipid-based Nano-formulation for resistant infectious diseases and is looking forward for more challenges in the said field.

Abstract:

Management of invasive candidiasis in immune compromised patients exhibits a major challenge for the clinicians since, antifungal monotherapy becomes ineffective. Moreover, switching between new antifungal agents and drug combinations intensify the situation by creating parallel adverse effects and drug toxicities. Furthermore, combining two drugs in a unit dosage form bring pharmaceutical challenges. This in turn, decreases the pharmacoeconomic efficiency in treatment associated with drug resistant candidiasis. Hence, we suggest replacing drug-drug therapy with drug-active lipid therapy,wherein the active lipid is a synthesized aminolipid based multifunctional carrier (PFA 18) which exhibit combinatorial synergy with existing first line antifungal agents like fluconazole and polyenes such as amphotericin B against clinically isolated drug resistant Candida species. As suggested by our computerized simulation studies, this lipid forms a strong hydrophobic and van der Waals interactions with antifungal agents supporting its stabilization into a nanoemulsion system. Sensitivity testing against gene deleted S. cerevisiae library also represented a possibility of effect on mitochondrial outer membrane and cell membrane of yeast. These activities were confirmed by flow cytometric mitochondrial assay and topographical cryoimaging studies. In vitro cytotoxicity confirmed that PFA 18 alone and in combinations with fluconazole/ amphotericin B did not show any cytotoxic effect on Hep2G or HEK cell lines. Moreover, in vivo acute toxicity testing of PFA 18 showed that its toxicity range lies between 200-300 mg/kg. In vivo efficacy studies in swiss albino mice model infected with multidrug resistance candida revealed that, PFA 18 played a major role in reducing drug dose, and toxicity thereby increasing the probability of survival.